• Scraping for Cytology and Cultures
o The cultures should be done before any drops, (drugs contain bacteriostatic agents)
o Topical anesthetics and the handle end of a scalpel blade to scrape.
o Sterile dacron swabs for culture.
• Culture cornea BEFORE applying anything to the eye if overt infection is suspected
• Fluorescein stain every eye that is PAINFUL!
o Corneal opacities
• Rose Bengal Stain
o Viral Keratitis
o Early Fungal Keratitis
o Assess tear film
• Deep corneal scrapings, at the edge and base of the ulcer to detect bacteria and fungal hyphae
• Obtained with topical anesthesia and the blunt, handle end of a sterile scalpel blade.
• Superficial swabbing cannot be expected to yield fungi in a high percentage of cases.
• Deep (Stromal)
• Infectious (fungal, bacterial, viral)
• Response to previous treatments
• Epithelium only
• Foals (Increased incidence compared to adults)
o Corneal sensitivity in foals is very poor.
o Lower tear production in foals compared to adult's
• Epithelium and collagen is lost
• Variation of the clinical picture
o Epithelium and stroma are not present
o Buldge of DM into the defect